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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be observed in several tumors, which results in chromosome instability, and therefore may lead to tumorigenesis. This protein was uncovered to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a essential part in advertising mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually explained. Two extra splice variants were being noted but detailed nucleotide sequence facts just isn't nonetheless offered.
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Blocking peptides are peptides that bind specially to the focus on antibody and block antibody binding. These peptide normally has the epitope recognized with the antibody.
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This manual outlines further probable optimization of mobile and immunoassay parameters to make certain the best possible success are...
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This manual outlines more probable optimization of mobile and immunoassay parameters to be sure the best possible benefits are...
Antibodies certain to the blocking peptide now not bind to the epitope within the goal protein. By comparing the staining from your blocked antibody compared to the antibody alone, one can see which staining is particular.
Focus on Facts Cyclin E belongs to your really conserved cyclin family, whose customers are characterized click here by a extraordinary periodicity in protein abundance through the cell cycle. Cyclins functionality as regulators of CDK kinases. Unique cyclins show distinctive expression and degradation patterns which contribute to the temporal coordination of each mitotic party. Cyclin E forms a posh with and functions being a regulatory subunit of CDK2, whose activity is needed for mobile cycle G1/S changeover. This protein accumulates in the G1-S period boundary and is also degraded as cells development by means of S stage.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both antibodies...
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